Formation and stability of repairable pyrimidine photohydrates in DNA
1990
Ultraviolet irradiation of poly(dG-dC) and poly(dA-dU) in solution produces pyrimidine hydrates that are repaired by bacterial and mammalian DNA glycosylases. Escherichia coli endonuclease III was used to quantitate the formation and stability of these hydrates in the double-stranded alternating copolymers poly(dG-dC) and poly(dA-dU). When poly(dG-dC) was irradiated with 100 kJ/m{sup 2} of 254-nm light at pH 8.0, 2.2{percent} of the cytosine residues were converted to cytosine hydrate (6-hydroxy-5,6-dihydrocytosine) while 0.09{percent} were converted to uracil hydrate (6-hydroxy-5,6-dihydrouracil). To measure the stability of these produces, poly(dG-dC) was incubated in solution for up to 24 h after UV irradiation. Cytosine hydrate was stable at 4 {degree}C and decayed at 25, 37, and 55 {degree}C with half-lives of 75, 25, and 6 h. Uracil hydrate produced in irradiated poly(dA-dU) was stable at 4{degree}C and at 25 {degree}C and decayed with a half-life of 6 h at 37 {degree}C and less than 0.5 h at 55 {degree}C. Uracil hydrate and uracil were also formed in irradiated poly(dG-dC). These experiments demonstrate that UV-induced cytosine hydrate may persist in DNA for prolonged time period and also undergo deamination to uracil hydrate, which in turn undergoes dehydration to yield uracil. The formation and stability of these photoproducts inmore » DNA may have promoted the evolutionary development of the repair enzyme endonuclease III and analogous DNA glycosylase/endonuclease activities of higher organisms, as well as the development of uracil-DNA glycosylase.« less
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
43
References
68
Citations
NaN
KQI