A Study on Chitinase Production from Bacillus licheniformis H-89

This research was to investigate optimal condition for effective chitinase production from Bacillus licheniformis H-89 by changes using working volume, carbon source, nitrogen source, culture temperature, and initial pH of medium at flask for 48 hr. The chitinase production was appropriate when the working volume was 50 mL/500 mL of flask. Among various carbon sources, glucose was best carbon source. Especially, 20 or 25 g/L of glucose was optimum concentration for effective chitinase production. Among various nitrogen sources, yeast extract was also best one for chitinase production. The optimal pH and optimal temperature of chitinase production ranged of 6.0 to 7.0 and 35℃. These results suggest that all these optimal conditions can be applied for effective chitinase production from Bacillus licheniformis H-89.