Tumor organoid model

The invention relates to a method of generating an artificial 3D tissue culture of a cancer grown in non-cancerous tissue, comprising the steps of providing an aggregate of pluripotent stem cells or progenitor cells, culturing and expanding said cells in a 3D biocompatible matrix, wherein the cells are allowed to differentiate to develop the aggregate into a tissue culture of a desired size; wherein at least a portion of said cells are subjected to cancerogenesis by expressing a oncogene and/or by suppressing a tumor suppressor gene during any of said steps or in the tissue culture, and further comprising the step of allowing said cells with an expressed oncogene or suppressed tumor suppressor to develop into cancerous cells; drug screening methods; oncolytic virus screening methods; a 3D tissue culture; and a kit for performing the inventive methods.