Fluorimetric determination of reducing carbohydrates with malonamide

Abstract Samples are heated for 90 min with malonamide in carbonate buffer (pH 9.2) for measurement at 383 nm (excitation)/425 nm (emission). For D-glucose, relative standard deviations are 1–3% at the 10 -6 –10 -9 mol level. Relative fluorescence intensities are reported for 36 carbohydrates.