Ion-pair LC―UV Method for the Determination of Boanmycin in Mouse Plasma and Its Application to a Pharmacokinetic Study

An ion-pair LC–UV detection assay was developed for the determination of boanmycin (BAM) in mouse plasma. The mobile phase contained methanol, acetonitrile, 0.055 M sodium hexanesulfonate and 0.08 M acetic acid in water, with a flow rate of 0.8 mL min−1. This assay involved a rapid and simple extraction process and subsequent detection at 291 nm. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.04 and 0.09 μg mL−1, respectively. Using bleomycin (BLM A5) as the internal standard, the assay was linear over the concentration range from 0.1 to 30 μg mL−1 (r 2 > 0.99). The extraction recovery of BAM in mouse plasma was >66.5%, and the intra- and inter-day accuracy and precision were ≤7.5%. The assay was successfully applied to a pharmacokinetic study after intravenous injection of BAM (40 mg kg−1) to mice.