AB1125 Urinary soluble cd25 as a biomarker of active lupus nephritis in egyptian children with juvenile systemic lupus erythematosus

Background Lupus nephritis (LN) is more prevalent and severe in children than adult and considered a major predictor of poor outcome. Thus, early diagnosis and treatment is associated with better outcome. Soluble CD25 (sCD25), also known as Interleukin-2 receptor alpha chain, is a type I transmembrane protein present on activated T lymphocytes that play important role in the pathogenesis LN[.1 Objectives This study aimed to measure urinary levels of sCD25 in children with juvenile systemic lupus erythematosus (JSLE) and to investigate its role as a potential biomarker of activity in LN. Methods We measured sCD25 using enzyme-linked immunosorbent assay in urine samples from 53 JSLE patients and in urine samples from 30 healthy controls and these levels were normalised to creatinine excretion in urine. All JSLE patients underwent thorough clinical examination and disease activity assessment using Systemic Lupus Erythematous Disease Activity Index (SLEDAI). Systemic Lupus International Collaborating Clinics (SLICC) renal activity score2 was used to assess activity of LN. Results Urinary sCD25 normalised levels were highly significantly increased in JSLE patients (278.38±133.68 pg/mg) compared to urinary level in the healthy controls (187.33±83.59 pg/mg),p Conclusions JSLE patients have significantly increased urinary levels of sCD25 especially in those with active LN. Urinary sCD25 levels are remarkably correlated with the renal disease activity scores suggesting that it could be a useful marker to reflect active renal involvement in JSLE patients. References [1] - Gupta R, Yadav A, Misra R, Aggarwal A. Urinary sCD25 as a biomarker of lupus nephritis disease activity. Lupus2015; Mar;24(3):273–9. [2] - Petri M, Kasitanon N, Lee SS, Link K, Magder L, Bae SC, et al. Systemic Lupus International Collaborating Clinics (008) Systemic lupus international collaborating clinics renal activity/response exercise. Development of a renal activity score and renal response index. Arthritis Rheum2008; 58(6):1784–88. Disclosure of Interest None declared