Correlation of single nucleotide polymorphisms of hTERT gene of 5'-upstream with susceptibility to lung cancer

Objective To study the association between single nucleotide polymorphism (SNP) of human telomerase reverse transcriptase (hTERT) gene of 5'-upstream sequence and lung cancer.Methods From January 2011 to December 2012,blood samples and clinical data of 154 patients with lung cancer newly diagnosed by histopathology in the Second Hospital of Lanzhou University were collected,including 116 males and 38 females.Mean age was (57.3 ± 11.6) years old.There were 45 adenocarcinomas (29.2％),55 squamous carcinomas (35.7％),and 54 small cell lung cancers (35.1％).Matching controls included 174 males and 48 females,aging (56.4±8.4) years old.The genotypes of SNP (rs2735846,rs2735948,rs62329683,rs2736106,rs3888705,rs2736108,rs2736109,and rs2735940) in hTERT gene were determined with ABI Prism SNaPshot.And then Hardy-Weinberg equilibrium test,the allelic frequency,and odds ratio were calculated.Results Except rs2736106,other seven SNP of hTERT were in Hardy-Weinberg equilibrium in control group.In dominant model,the CG + CC and GG distribution of rs2735846 in lung cancer patients was significantly different from that in controls.Conclusions The results suggests that the rs2735846 SNP in 5'UTR of hTERT gene is associated with the risk of lung cancer.The allele C is the risk factor of lung cancer. Key words: Telomerase reverse transcriptase;  Single nucleotide polymorphism;  Lung cancer;  Genetic predisposition to disease