In vitro organogenesis from adult tissue of ‘Bahia’ sweet orange (Citrus sinensis L. Osbeck)

Introduction . Plant regeneration from in vitro culture of adult citrus tissue may help in early evaluation of important horticultural characteristics of genetic material from breeding programs, which incorporate biotechnological tools. Our research aimed to optimize in vitro culture conditions that could lead to the maximum plant regeneration through organogenesis of ‘Bahia’ sweet orange ( Citrus sinensis L. Osbeck) in explants (internodes and foliar disc segments) collected from adult plants grown under greenhouse conditions. Materials and methods . Organogenesis was induced in internodes on DBA3 culture media supplemented with BAP at (0.0, 1.0, 2.0 and 3.0) mg·L –1 combined with NAA (0.5 mg·L –1 ), and in foliar discs on culture media with BAP at (0.0, 0.5, 1.0, 1.5 and 2.0) mg·L –1 combined or not with NAA (0.5 mg.L –1 ). Results and discussion . The concentrations of (1.0, 2.0 or 3.0) mg·L –1 of BAP combined with 0.5 mg·L –1 of NAA induced in vitro organogenesis in internodes of ‘Bahia’ sweet orange. The use of foliar discs was not efficient for adventitious organogenesis.