Effects of PEG and NaCl stress on the contents of indigotin and indirubin in Isatis indigotica tetraploids

Objective: To provide a reference evidence for choosing salt-resistent and drought-resistent varieties of Isatis indigotica Fort..Methods: The tissue culture seedlings of different Isatis indigotica tetraploids were used as plant materials.The diploid was used as CK,which were cultured for 30 days.2 g / L NaCl and 15% PEG-6000 were used as stress treatment respectively for 12 hours,then poured out the solution,the materials were continuously cultured for 7 days.The contents of indirubin and indigotin were determined by HPLC.Results: The contents of indigotin and indirubin of DB3,DB5 and DB12 with PEG treatment and DB2,DB3 and DB12 with NaCl treatment were increased.Conclusion: DB3 and DB12 can be further studied as reproduction materials.