GAMMA RAYS INDUCED PROTEIN VARIATIONS IN M 4 GENERATION OF BLACK GRAM ( VIGNA MUNGO L.)

Effects of various doses (45 krad, 55 krad and 65 krad) of gamma rays on proteins of black gram (Vigna mungo L.) in M 4  generation were investigated using Sodium dodecyl sulphate-poly acrylamide gel electrophoresis (SDS-PAGE) analysis. Higher doses of gamma rays resulted in reduced seed germination and affected further plant growth. SDS-PAGE results indicated differences in the protein banding patterns in 45 krad, 55 krad and 65 krad irradiated black gram samples. Absence of protein bands and appearance of new protein bands in M 4 generation black gram indicate the gamma rays-induced genomic lesions corresponding to these missing protein bands which could not be repaired even after M 4 generation and was quite stably inherited in M 4 generation. This study provides the useful information for the use of these black gram mutant lines in future breeding programme and further agronomic and nutritional study is needed for the analysis of the mutants. Keywords : Black gram; gamma irradiation; SDS-PAGE; Vigna mungo.