[Influence of different preparation on the content of carbohydrate and physicochemical properties of polysaccharides from raw paeoniae radix alba and stir-baked paeoniae radix alba].

Objective: To observe the difference of two preparation methods on the content of carbohydrate and physicochemical properties of polysaccharides from raw Paeoniae Radix Alba and stir-baked Paeoniae Radix Alba. Methods: Polysaccharides extracted from raw Paeoniae Radix Alba and stir-baked Paeoniae Radix Alba with water were precipitated by ethanol and named as BSEP-S and BSEP-C,respectively. In the same way,those deposited by acetone were named as BSAP-S and BSAP-C. Their physicochemical properties,including extraction yield,the content of carbohydrate,elemental composition and monosaccharide composition,were determined. Results: Extraction yield,sugar content and uronic acid content of BSEP-S was 1. 56%,80. 56% and 3. 33%,BSEP-C1. 18%,80. 79% and 5. 47%,BSAP-S 1. 58%,86. 50% and 3. 79%,and BSAP-C 1. 54%,81. 64% and 3. 25%,respectively. In addition, monosaccharide compositions showed that glucose was the main monosaccharide and successively reached 92. 21%,87. 30%,91. 45% and 86. 62%. Conclusion: Yield and content of carbohydrate from raw Paeoniae Radix Alba and stir-baked Paeoniae Radix Alba by water extraction-ethanol precipitation are a little higher than that by water extraction-acetone precipitation,but monosaccharide compositions are almost the same. Different preparation has significant impact on the yield and the content of carbohydrate in Paeoniae Radix Alba by stir-baked method,and it can decrease the dissolution of polysaccharide.