Simultaneous determination of eight active components in Chrysanthemum indicum by HPLC

This study is aimed to establish a high-performance liquid chromatography(HPLC) method for simultaneous determination of chlorogenic acid,caffeic acid,1,3-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid,luteolin-7-O-β-D-glucoside,3,4-dicaffeoylquinic acid,linarin and luteolin in Chrysanthemum indicum.The separation was carried out on a Shim pack VP-ODS(4.6 mm×250 mm,5 μm) column eluting with mobile phases of methanol(A) and water containing 0.3% phosphoric acid(B) in gradient mode(0-9 min,85%-80%B;9-12 min,80%-70%B;12-15min,70%-65%B;15-20 min,65%-60%B;20-23 min,60%-55%B;23-29 min,55%-54.4%B;29-32 min,54.4%-45%B;32-37 min,45%-5%B;37-45 min,5%-85%B) at the flow rate of 1.0 mL·min-1.The column temperature was 35 ℃ and the detection wavelength was set at 326 nm.The good separation of chlorogenic acid,caffeic acid,1,3-dicaffeoylquinic acid,3,5-dicaffeoylquinic acid,luteolin-7-O-β-D-glucoside,3,4-dicaffeoylquinic acid,linarin and luteolin was achieved within 40 min.Calibration curves of the eight effective components showed good linear relationship(r0.999 5,n=7).The average recoveries were within 97.03%-102.3%(RSD2.0%,n=6).The method is simple,accurate and repeatable and can be used for the quality control of Ch.indicum.