[The effect of anthocyanins on cholesterol efflux from mouse peritoneal macrophage-derived foam cells and its possible molecular mechanism].

Objective To investigate the potential role of anthocyanins on modulating cholesterol efflux in mouse peritoneal macrophage-derived foam cells and related molecular mechanisms.Methods The macrophages were isolated from pathogen-free NIH mice and were loaded with 50 μg/ml oxLDL for 24 hours, newly formed foam cells were then treated with anthocyanins (cyanidin-3-glucoside,Cy-3-g;or peonidin-3- glucoside,Pn-3-g) at the concentrations of 1 μmoL/L,10 μmol/L,100 μmol/L for 0 to 36 hours, respectively.The enzymatic-fluorescent method was used to determine cholesterol content in culture medium. ABCA1 expressions at mRNA and protein level were detected by real-time PCR and confocal microscope. Results Cholesterol efflux of macrophage-derived foam cells increased in a time-and dose-dependent manner post anthocyanins treatment.ABCA1 expressions at mRNA and protein levels were also significantly enhanced after anthocyanins treatment in these cells and these effects could be blocked by co-treatment with DIDS,an inhibitor of the transport activities of ABCA1 and blocker of apoAI-mediated cholesterol efflux. Conclusion These data demonstrate that anthocyanins induce cholesterol efflux from mouse peritoneal macrophage-derived foam cells via regulating ABCA1 expression.