Isolation, identification and characterization of pancreatic lipase inhibitors from Trigonella foenum-graecum seeds

ABSTRACT Activity-guided fractionation of the methanol extract of Trigonella foenum-graecum seeds furnished three flavone C-glycosides having pancreatic lipase inhibitory activity. Structures of these compounds were established as vicenin-1 ( 1 ), isoschaftoside ( 2 ) and schaftoside ( 3 ) by detail analysis of 1 H, 13 C NMR and MS data. These compounds showed percentage inhibition 60.3% ( 1 ), 33.8% ( 2 ) and 95.5% ( 3 ) at the concentration of 250 μg/ml and IC 50 values of the inhibition were 207 μg/ml ( 1 ), 330 μg/ml ( 2 ) and 130 μg/ml ( 3 ). This is the first report of the isolation of lipase inhibitors from T. foenum-graecum seeds. Temperature dependency of 1 H NMR of 3 was studied in detail.