Expression of caveolin-1 and the invasion of choriocarcinoma

Objective To investigate the association between the expression of caveolin-1(CAV-1) and the invasion of choriocarcinoma,and to explore the effect of CAV-1 small interfering RNA(siRNA) on the invasion of choriocarcinoma cell line JEG-3.Methods(1) Matrigel invasion assay and 3-(4,4)-dimethylthiahiazo(-z-yl)-3,5-di-phenytetrazoliumormide(MTT) assay were used to examine the difference in invasion and proliferation ability between JEG-3 cells and JAR cells;(2) Expression of caveolin-1 gene in the human chorionic villi tissues and chorionicnoma cell lines(JEG-3 cells and JAR cells) were detected by semi-quantitative RT-PCR.(3) The effect of CAV-1 siRNA transfection on the expression of CAV-1 mRNA,and the invasion and proliferation ability of JEG-3 cells were measured by RT-PCR,Matrigel invasion assay and MTT assay.Results(1) The invasion ability of JEG-3 cell line was stronger than that of JAR cell line(P0.05),but the difference in proliferation ability between JAR and JEG-3 was not obvious(P0.05);(2) The expression of caveolin-1 gene in chorionicnoma cell lines was significantly stronger than that in the human normal chorion(P0.05),and the expression of caveolin-1 gene in JEG-3 cells was stronger than that in the JAR cells(P0.05).The data suggested that there was significantly positive correlation between caveolin-1 and the invasiveness of chorionicnoma cells(r=0.086,P0.05);(3) CAV-1 siRNA could knock-out the expression of CAV-1 mRNA,and inhibit the invasion and proliferation ability of chorionicnoma cells.Conclusion CAV-1 can promote the invasion ability of chorionicnoma cells.CAV-1 siRNA can inhibit the invasion and proliferation ability of chorionicnoma cells.