Detection and typing of human papillomavirus by a GeXP based multiplex PCR assay

Objective To establish a new and rapid GeXP based multiplex PCR assay for the detection and typing of human papillomavirus 6, 11,31,33 and 52. Methods Nucleotide sequences of HPV6, HPV11, HPV31, HPV33 and HPV52 from NCBI were obtained and compared. Genotype-specific primers were then designed and the sensitivity and specificity of multiple PCR assay was evaluated.Optimized assay was further validated with 30 clinical specimens collected from the cervical secretions of patients. Results A GeXP based multiplex PCR was developed for sensitive detection and reliable differentiation of five HPV genotypes( HPV6,11,31,33 and 52) , Conclusion A GeXP based multiplex PCR assay is demonstrated to be a new and rapid technique for simultaneous detection and typing of 5 different human papillomaviruses. Key words: Polymerase Chain reaction; Papillomavirus, human; Genotype