치주염 및 임플란트 주위염 환자의 치은 조직에서 Real Time PCR을 이용한 Matrix Metalloproteinase (MMP)-2,9,12,13의 발현

Matrix metalloproteinases (MMPs) are known to be responsible factor in various pathological conditions including stomach ulcer, tumor metastasis, rheumatoid arthritis. In previous study, MMP-8 is kown as a key mediator in periodontitis but the exact role and functions of MMPs are still unclear. This study was to investigate the expressions of MMPs in gingival tissues from periodontally healthy individuals, chronic periodontitis patients, periimplantitis patients using real time polymerase chain reaction analysis. MMP-2, MMP-9, MMP-12, MMP-13 were analyzed in this study. mRNA level of MMP-2, MMP-9 were high in tissue extract isolated from advanced periodontitis compared to that of periodontally healthy individuals. Unlike to periodontitis, in peri-implantitis patients, only MMP-9 was slightly activated compared to periodontally healthy individuals. Otherwise, expression level of MMP- 12, MMP-13 in periodontally unhealthy patients was extremely low. In RNA level, expression profile of MMPs between periodontitis and peri-implantitis was similar but there are some different aspects in individual expression. It can imply that there be the similar mechanisms and relation between two diseases but different regulatory mechanisms can be exsist.