Short Sequence-Paper Cloning and expression of a cDNA encoding bovine muscarinic acetylcholine m3 receptor

A cDNA clone encoding bovine muscarinic acetylcholine m3 receptor has been isolated from a bovine brain library. Sequencing of this clone has revealed a single open reading frame encoding a protein of 590 amino acids. Comparison with known muscarinic m3 receptor sequences from other species shows a high degree of conservation (92-98% homology) in the protein sequence. COS ceils transfected with this cDNA expressed a single high-affinity (K d = 43.9 pM) binding site for muscarinic receptor ligand N-[3H]methylscopolamine.