2P-167 : Antioxidant Agent Loaded Nanopaticle Using pH Gradient Loading Method to Enhance Stability, Antioxidation Effect

Reactive oxygen species (ROS) generation occurs oxidative stress to cells. It makes cell aging, disorder and death. Thus there are many studies to remove ROS using antioxidant agents. Researchers want to apply these agent in physiological environment. However, some of the antioxidant agents are unstable in a physiological environment (pH7.4) and easily destroyed during formulation. These problems should be solved for bio-application of antioxidant agents. To preserve their antioxidant ability, we used nanoparticle to formulate them. It makes more stable in pH 7.4 compare to unformulated antioxidant agents. Furthermore we tried another loading method, rather than passive loading. It comes 2-folded high loading efficiency of nanoparticle. Also, in release test, loaded antioxidant agents release slowly over 14 days. It means that antioxidant capacity maintain for a long time. Consequently, antioxidant agent loaded nanoparticle is effect on stability in physiological environment.