Construction and Expression of Recombinant Plasmid Co-expressing Myostatin and Inhibin Genes

Two DNA fragments encoding N-term of myostatin ( MSTN ) and inhibin ( IHN ) were incorporated into Hepatitis B virus S gene at C-term and site of amino acid sequence 112 and 113 to construct an expression plasmid of pVAX- SMI. The plasmid pVAX- SMI was identified by restriction endonuclease digestion and sequencing. pVAX- SMI was then transfected into HeLa cells and the recombinant protein was detected by Western blot. The result showed that the plasmid could express recombinant MSTN and INH successfully. And the pVAX- SMI expression plasmid was constructed successfully.