Characteristic chromatogram of volatile oil in Ganmao Qingre granules and determination of menthone and pulegone

Objective:To establish the characteristic chromatogram of volatile oil in Ganmao Qingre granules and determine the contents of menthone and pulegone in it.Methods:Gas chromatography was used and the conditions were as follows:Agilent HP-5 capillary column(30 m×320 mm,0.25 μm);injection port and detector temperatures were 250 ℃;the initial column temperature was 40 ℃ maintained for 1 min,then 7 ℃·min-1 up to 100 ℃ maintained for 12 min,3 ℃·min-1 up to 150 ℃ maintained for 8 min,finally 10 ℃·min-1 up to 240 ℃ maintained for 2 min;the carrier gas was nitrogen and its flow rate was 1 mL·min-1,splitless.Results:There were 6 characteristic peaks in the GC characteristic chromatograms.The good linear relationship(r=0.9999) for menthone and pulegone were 0.0211-0.422 μg and 0.0371-0.743 μg,respectively.The average recoveries were 101.0% and 97.5% with the RSD of 2.6% and 2.1%,respectively.Conclusion:The established GC characteristic chromatogram and the determination method can be used to control the volatile components in Ganmao Qingre granules qualitatively and quantificationally.The method is accurate and feasible,and can be used for quality control of Ganmao Qingre granules.