Effect of Chaihu Shugan Powder and Xiaoyao Powder on Serum Corticosterone and Gastrointestinal Hormones of Chronic Multi-stress Rats

Objective To observe the content changes of corticosterone and gastrointestinal hormones in serum of chronic multi-stress rats and to investigate the effect of Xiaoyao Powder and Chaihu Shugan Powder.Methods Wistar rats were randomly allocated to four groups:normal group(group A),model group(group B),Xiaoyao Powder group(group C),Chaihu Shugan Powder group(group D)etc.Chronic multi-sress models were established in all the rats except those of normal group.Xiaoyao Powder group and Chaihu Shugan Powder group were respectively given the corresponding drugs for 21 days.Normal group and model group were administered the equal amount of normal saline by gavage.After the administration,the open-field test was used to observe the behavior changes of the rats,and the radioimmunoassay method was adopted to detect the serum corticosterone and gastrin contents and plasma motilin level.Results Compared with the normal group,the body weight of model group was decreased(P 0.01)and the open-field behavior frequency was reduced(P 0.01),the corticosterone content in serum increased(P 0.01),and gastrin in serum and motilin in plasma levels were decreased(P 0.01).Compared with the model group,the body weight and open-field behavior frequency in Xiaoyao Powder group and Chaihu Shugan Powder group were increased(P 0.05,P 0.01),the open-field behavior frequency increased(P 0.05,P 0.01),the corticosterone content in serum decreased(P 0.01),and the gastrin in serum and motilin in serocym levels increased(P 0.05).Conclusions Chronic multi-stress can result in rats growth restraining,abnormal mental state and gastrointestinal dysfunction through increasing corticosterone and decreasing gastrin and motilin.Chaihu Shugan Powder and Xiaoyao Powder can restrain the influence of chronic multi-stress by regulating those hormones levels in rats body.