Establishment of two induced pluripotent stem cell line from healthy elderly (IPTi005-A and IPTi007-A)

Abstract Two healthy elderly donated their peripheral blood mononuclear cells (PBMCs). The induced pluripotent stem cell lines (IPTi005-A and IPTi007-A) were reprogrammed by episomal vector system with Yamanaka factors OCT4, SOX2, C-MYC and KLF4. Both the two iPSC lines can differentiate into three germ layers determined by embryoid bodies (EBs) differentiation. Flow cytometry analysis showed that more than 90% cells expressed NANOG, OCT4 and SSEA4. Besides, the iPSC lines of IPTi005-A and IPTi007-A were confirmed to exhibit a normal karyotype. In the studies of age-related disease, the iPSC lines can be used as controls.