Evaluation the Effects of Using of Probiotic, Enzyme and Pennyroyal Herb on Performance of Laying Hens

Plants and plant parts have an indispensable source of medicine for indigenous poultry production systems. On the one hand, the gastro intestinal tract of poultry harbor a micro flora which is formed immediately after the bird is born and is an important barrier against colonization of potentially pathogenic microorganisms. As the part of study, the effects of Probiotic (Pr) enzyme (En) and Menthapulegium L. (Pennyroyal, MpL.) on performance of laying hens was evaluated. Hy-Line hens (n=432), 34 wk old, were randomly housed in windowed cages in a completely randomized design with factorial arrangement of 3×2×2 with twelve treatments and three replicates (12 birds per each) and were fed 1 of 12 diets: control (treatment 1, T1), En, (T2), Pr (T3) and En + Pr (T4), 1% MpL. (T5), 1% MpL. + En (T6), 1% MpL. + Pr (T7), 1% MpL. + En + Pr (T8), 2% MpL. (T9), 2% MpL. + En (T10), 2% MpL. + Pr (T11), 2% MpL. + En + Pr (T12) throughout 12 wk experimental feeding period. Diets supplemented according tothe manufacturer'srecommendation with amounts of En (0.05%), Pr (0.005%) and a combination of both, respectively. Results indicated that individual treatments (T1, T2, T3 and T5) could not improve performance of laying hen. All interactions between experimental supplements (En, Pr and different levels of MpL.) not affected all performance parameters (feed intake, FCR egg production percent and egg mass) with the exception of interaction between 1% MpL., En and Pr (T8) (P>0.01) which significantly elevated feed intake but decreased FCR and egg production. From the results of the present study, it was concluded that combination of 1 and 2 % Pennyroyal individually, or in combination with Probiotic, enzyme and both could not provide an improved performance of laying hens.