Covalent coupling of unsaturated compounds to thiol agrarose using γ-Radiation

Unsaturated compounds can be attached to thiol polymers by means of ΰ-radiation. By this method several agarose conjugates have been synthesized from thiol agarose gel and different ligand derivatives (testosterone, estradiol, 3-octenoic acid, and hexa-2,4-dienoic acid). The amount of fixed ligand is about 60-120 μmol/g of dried conjugate. The method described is a valuable complement to the currently available procedures for the preparation of adsorbents for affinity chromatography.