缺血后处理对在体大鼠缺血－再灌注心肌细胞色素P450表氧化酶2J3/环氧二十碳三烯酸系统的影响

Objective To observe the variation of endogenous CYP2J3/EET system in rat heart in vivo after ischemic postconditioning (IPo). Methods All Wistar rats were randomly divided into three groups as follows: Sham group, I/R group and IPo group. The myocardial ischemia-reperfusion and the IPo models in vivo were made. Observing the changes of heart rate (HR), left ventricular end-systolic pressure (LVESP) and maximum velocity of increase or decrease of LV pressure (±LVdP/dt(subscript max)). Infarction size was measured by triphenyltetrazolium chloride (TTC) staining. The expression of CYP2J3 mRNA was tested by RT-PCR. The expression of CYP2J3 protein was detected by Western blot. 11, 12-epoxyeicosatrienoic acid (11, 12-EET) was observed by high-performance liquid chromatography (HPLC). Results Compared with I/R group, the levels of LVESP and ±LVdp/dt(subscript max) were all significantly increased. Infarction size was significantly reduced in IPo group compared with I-R group (P＜0.05). CYP2J3 mRNA was increased compared with I/R and Sham groups, consistent with increased expression of CYP2J3 protein and 11, 12-EET. Conclusion These data suggest that IPo protects myocardium against I-R injury which may involve endogenous activation of CYP2J3/EET system.