沙枣根瘤内弗兰克氏菌分离鉴定及生态特性研究 Isolation and Ecological Characteristics Research of Frankiaceae in Elaeagnus angustifolia L. Root Nodule

黑龙江省苏打盐碱地在生物治理过程中成功地引种沙枣进行生物修复。由于沙枣能够形成放线菌根瘤，极大的提高了其抗逆性。本研究采用根瘤切片法对沙枣根瘤内的放线菌进行分离，并通过分子生物学手段进行鉴定，共得到两种Frankia (Frankia sp1、Frankia sp2)，同时还获得了Micromonospora sp.和Nocardia sp.各一种；Frankia sp1属于生理A型，而Frankia sp2属于生理AB型；Frankia sp1和Frankia sp2均具有较强的耐盐能力，且Frankia sp2优于Frankia sp1；两种Frankia在pH值为7.5时菌株的生长量达到最大。研究结果进一步充实了沙枣根瘤研究的理论基础。 We successfully introduced E. angustifoliato conduct biological repairing in soda salinization soil of Heilongjiang Province, China. Resistance of E. angustifolia has been greatly improved because of the formation of Actinomycetes nodule. In this study, Actinomycetes has been separated from E. angustifolia nodule by the method of nodules sectioning and identified by the method of molecular biology, which has obtained 2 Frankiastrains (Frankia sp1 and Frankia sp2). At the same time, we have obtained one of Micromonospora sp. and Nocardia sp. Frankia sp1 belongs to physiological type A, Frankia sp2 belongs to physiological type AB. Both of them showed strong salt tolerance, but Frankia sp2 was better than Frankia sp1. Two kinds of Frankia strain growth maximum at a pH of 7.5. The results enrich the theoretical basis of the research of E. angustifolia root nodules.