DESARROLLO DE UN PROTOCOLO PARA PROPAGACION IN VITRO DE ROBLE (Tabebuia rosea Bertol DC) DEVELOPMENT OF AN IN VITRO PROPAGATION PROTOCOL FOR ROBLE (Tabebuia rosea Bertol DC)

A significant advance for oak (Tabebuia rosea) micropropagation has been developed in order to massively propagate clones. The effect of three sodium hypochlorite concentration (1%, 2% and 3%) at three times (5, 10 y 15 min) on surface disinfestations were evaluated using explants consisting of 2-3 cm axillary shoots established on ½MS with (in mg L -1 ) mio inositol (100), sucrose (30,000), thiamine HCl (0.4) and TC agar (8,000) (Sigma Co.). Five BAP (0.00, 2.22, 4.44, 8.88, 17.76 µM) concentration were