Determination of fat in leather by the use of supercritical fluid extraction combined with on-line piezoelectric detection
2001
Leather samples were prepared and characterized as ‘in
house’ matrix standards for the determination of fat. The Soxhlet
standard method was used to establish the reference fat content in every
standard sample. Sample homogeneity and stability were examined under
specific storing conditions. The materials were subsequently used as matrix
standards for the determination of fat in leather samples, using
supercritical fluid extraction (SFE) with on-line piezoelectric detection.
Real samples were weighed in the extraction SFE thimble, previously loaded
with 1 g of diatomaceous earth. A temperature of 45 °C and a
CO2 fluid density of 0.85 g ml−1 were used for
extraction. The linear calibration range thus achieved was
0.001–0.040% m/m total fat (related to the weight of the leather) and
the relative standard deviation ±3% (n = 11; P =
0.05). The results were compared with those obtained with the Soxhlet
method and no significant differences were found.
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