Pre-Study on the Molecular Mechanism of Self-Compatibility in Longtanzhenzhuli

Longtanzhenzhuli which is a new variety of plum with high quality were used as the experimental materials in this research. A specific primer was designed based on the conserved sequences of plum (Prunus salicina Lindl.) S-Rnase(S) genes, two specific fragments were isolated from Longtanzhenzhuli genome DNA with tese specific primers. The sequencing results showed that these fragments were confirmed as the plum S genes. One was first cloned from Prunus salicina and named PsS-RNase-28, with length of 727 bp, containing 367 bp intron and 360 bp Exton. Comparing with Prunus spinosa S-RNase S1 gene (EF636467.1), it showed high identity (98%), more than 79% with other plums S-RNase genes. The other fragment with 718 bp in length, containing 343 bp intron and 375 bp Exton, showed high identity (99%) with Prunus salicina PsS-RNase-h gene (AB084148.1), and more than 85% with other plums S-RNase genes. And sequences follow-up analysis of these fragments would be benefited to study on the mechanism of self-compatibility of Longtanzhenzhuli.