Detection of Human Papillomavirus DNA in Squamous Papilloma of the Larynx by In Situ Hybridization

=Abstract=ln situ hybridization using digoxigenin labeled HPV(human papillornaviru­ ses) probes was tried in order to detect the presence of HPV DNA in 27 cases of single and multiple laryngeal papilloma. There were HPV6/11 DNA signals in 17 out of 22 cases of multiple laryngeal papilloma and in all five cases of single laryngeal papilloma. The specific signals were seen focally in the nuclei of superficial epithelial cells. None of the cases was positive for HPVI6/18. There was no cross hybridization between HPV6/11 and HPV16/18 under the stringent conditions used in these experiments. So it could be said that HPV6/11-related sequences were found in laryngeal papillomas.