Software for Non-Parametric Image Registration of 2-Photon Imaging Data

Functional 2-photon microscopy is a key technology for imaging neuronal activity which can, however, contain non-rigid movement artifacts. Despite the established performance of variational optical flow (OF) estimation in different computer vision areas and the importance of movement correction for 2-photon applications, no OF-based method for 2-photon imaging is available. We developed the easy-to-use toolbox Flow-Registration that outperforms previous alignment tools and allows to align and reconstruct even low signal-to-noise 2-photon imaging data.