Določanje klonalnosti limfoidnih proliferacij

At the Department of Molecular Diagnostics, a new method for determining lymphoid proliferation clonality has been introduced in lymphoma diagnostics. The method is based on PCR and on the use of various consensus primers. It is validated and registered for the use in clinical diagnostics (CE-marked method). Our measurements confirmed that the use of extended number of primers improved the sensitivity of determining the lymphoid clonality of B-lymphocytes and T-lymphocytes by 23.1% and 14.3%, respectively. Both methods may, theoretically, be used in determining monoclonal population of lymphocytes in 84.6% of B- and 85.8 of T-lymphomas.