Подбор условий проведения ISSR-ПЦР для сортовой голубики высокорослой ( Vaccinium corymbosum)

In the experiments, the effect of three variable factors in PCR mixture was assessed: concentration of MgCl 2, concentration of dNTP, and the amount of DNA template. The mixture was used for ISSR profiling of plants with primer UBC 818. The optimum parameters of PCR mixture were stated taking into account the higher number of ISSR fragments and sufficient amount of copies of each fragment. These parameters were (per 25 μl of mixture): 2-2.5 mM MgCl 2, 125 μМ dNTP mix (1 : 1 : 1 : 1), 10-20 ng of DNA template.