High performance capillary electrophoresis fingerprint of Corni Fructus pieces.

Objective To establish a micellar electrokinetic chromatography method with high performance capillary electrophoresis(HPCE) fingerprint of Corni Fructus pieces.Methods Separation was performed on a fused-silica capillary column(64.5 cm×75 μm,effective length 56 cm) with 50 mmol/L borax solution-40 mmol/L SDS-5% acetonitrile(pH 9.5) as HPCE buffer.The running voltage was 10 kV,the detection wavelength was 240 nm,and column temperature was 18 ℃.Taking loganin as reference,the data were analysed by Fuzzy Cluster and Fingerprint Similarity Evaluation Software to compare the similarity of samples.Results HPCE Fingerprint was established with nine common peaks.Conclusion The method is accurate,simple,and suitable to the quality control of Corni Fructus.