Optimal processing techniques of preparing evodiae juice by orthognal experiment

OBJECTIVE: To study and establish the optimal technology for the preparation of evodiae juice. METHOD: The contents of evodiamine, rutaecarpine and evodin were simultaneously determined with HPLC, and each yield of the three compounds were chosen as the evaluating indicator. The orthogonal test coupled with the weighted coefficient method were adopted to acquire the optimal technology for the preparation of evodiae juice. RESULT: The study showed that the optimal technology for the preparation of evodiae juice was as follows: decocted three times while the first time was with 12-fold of water socked 30 minutes and decocted 45 minutes, the second time was with 8-fold of water decocted 20 minutes and the third time was with 6-fold of water decocted 20 minutes. CONCLUSION: This method is simple and accurate. The optimal technology is suitable for industry manufacture of evodiae juice.