Relative intensities of recognition factors at two combining sites of Ralstonia solanacearum lectin (RSL) for accommodating LFucα1-->, DManα1--> and Galβ1-->3/4GlcNAc glycotopes.

Abstract Owing to the weak reactivities of monomeric d Manα1 and Galβ1→3/4GlcNAcβ ( I β / II β ) glycotopes with Ralstonia solanacearum lectin (RSL), their recognition roles were previously ignored. In this study, the interaction intensities of RSL toward four monomeric glycotopes l Fucα1→, d Manα1→ and I β / II β within two combining sites were established by both enzyme-linked lectinosorbent and inhibition assays. It was found that high density of l Fucα1→ complex enhanced the recognition intensities at l Fucα1→ site, polyvalent d Manα1→ was essential for binding at the d Manα1→ site and polyvalent I β / II β was required at l Fucα1→ site. The peculiar recognition systems of RSL are very different from other well known microbial lectins.