poly(caprolactone) nanofibers fabrication with different roughness to tissue engineering

Developing scaffolds that mimic the architecture of tissue at the nanoscale is one of the major challenges in the field of tissue engineering. Extracellular matrix fibers (ECM) are an important component in tissue and organ development. Nanofiber polymers could play the same critical role in tissue regeneration Process. Nanofiber polymers which compose scaffolds were investigated for potential application of skin tissue engineering. The present study reports the fabrication of poly(caprolactone) (PCL) scaffolds with different surface roughness via electrospining that characterized by scanning electron microscopy. Fibroblast cells behavior on scaffolds was studied by MTT assay and dapi. Herein, we hypothesized that using the PCL scaffolds with different surface roughness drives fibroblast cell into a morphology that induced cell behaviors modulation that yield maximum amount of attachment and proliferation. So based on the need, the proposed fiber skin substitutes can be successfully fabricated and optimized for skin fibroblast attachment and growth. Promising strategies are currently being investigated to allow for the fabrication of optimal polymer nanofiber tissue engineering scaffolds with the goal of treating damaged and degenerated tissues.