A Comprehensive Insight into the Protein-Surface Biomolecular Interactions on a Smart Material: Complex Formation between poly(N-vinyl caprolactam) and Heme Protein

Proteins are naturally occurring biopolymers that exhibit ample range of functional applications. Meticulous knowledge about biomolecular interactions between polymeric biomaterials and body fluids or proteins are essential for designing bio specific surfaces and understanding protein-polymer interactions beyond existing limitations. In this regards, we studied comparative effect of heme proteins such as cytochrome c (Cyt c), myoglobin (Mb) and haemoglobin (Hb) on the phase behavior of poly(N-vinyl caprolactam) (PVCL) aqueous solution and demonstrated various biomolecular interactions that are taking place between polymer-protein complex with aid of various biophysical techniques. Absorption spectroscopy, steady-state fluorescence spectroscopy, Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS) studies, laser Raman spectroscopy, field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM) were operated at room temperature to examine the changes in...