Temperature-dependent Ca2+ mobilization induced by hypoxia-hypoglycemia in the monkey hippocampal slices.

Abstract Mobilization of [Ca 2+ ]i in the monkey hippocampal slices during transient hypoxia-hypoglycemia and KCl-induced deporalization was analyzed by microfluorometric imaging and anti-PIP2 immunohistochemistry. Hypoxia-hypoglycemia provoked the largest [Ca 2+ ]i mobilization of CA-1 temperature-dependently whereas [Ca 2+ ]i mobilization by KCl-induced depolarization occurred independent of the temperature in CA-2. Immunohistochemical analysis of the hippocampus after hypoxiahypoglycemia showed an increased PIP2 staining preferentially in the perikarya of CA-1 neurons. These data suggest that release of Ca 2+ from intracellular stores caused by PIP2 breakdown may induce elevated [Ca 2+ ]i.