Engineering of Microbial Carbonic Anhydrase for Enhanced Carbon Sequestration

Biosequestration of carbon dioxide using microbial carbonic anhydrase (CA) is one of the most environmentally friendly and attractive biomimetic routes for carbon sequestration due to its economic viability and environmental compatibility. However, the optimum use of microbial CA in industrial applications is hindered by the cost and unstable nature of enzyme. The use of engineered microbial CA as a reaction promoter can overcome these obstacles. Engineered microbial CA can be used to accelerate the CO2 hydration rate with improved thermokinetic stability. This chapter introduces multiple possibilities for CA engineering methods to promote the enhancement of carbon dioxide conversion and economical use of microbial CA. To reduce the greenhouse effect of carbon dioxide, further research should be devoted to the large-scale production, long-term performance of engineered CA, and developing highly efficient engineering strategies.