Photodynamic effects of radachlorin on cervical cancer cells

4737 Photodynamic therapy (PDT) is a novel treatment modality that produces local tissue necrosis with laser light after prior administration of a photosensitizing agent. Radachlorin has been recently shown to be a promising PDT sensitizer. In order to elucidate the effects of PDT using Radachlorin on cervical cancer, growth inhibition studies on cervical cancer cell line, TC-1 cells in vitro and cervical cancer animal model in vivo were determined. TC-1 cells were exposed to Radachlorin and irradiated with 12.5, 25 and 50 J/cm 2 at an irradiance of 20mW/cm 2 with a Won-PDT D662 laser at 662 nm. C57BL/6 mice were injected different routes with Radachlorin of 40mgkg -1 body weight and carry out a PDT in vivo. The power density (2 W/cm 2 ) and the irradiation time (150 sec) were used. Growth suppression was then evaluated at various time periods after PDT. The results showed that irradiation of TC-1 tumor cells in the presence of Radachlorin induced the cell growth inhibition significantly. For the in vivo experiments, the tumor growth rates in the Radachlorin-only and irradiation-only groups were not significant to no-treatment group. But, the rates of tumor inhibition following treatment with the PDT was 63.33%, 55.56%, 57.90% and 55.13% by tumor mean diameters in comparison to control mice on day 2, 4, 6 and 10, respectively. (p