The relationship between the structure and immune enhancement of bursin like peptides

Three kinds of different structure of bursin like peptides were synthesized as single copy BS,30 copies tandem repeats(KG30) and 30 copies branch structure(MAP),to compare their immunity enhancement activities by the peripheral blood lymphocyte stimulate proliferation test,the antibody production of hybridoma cells stimulation test,the E-rosettes test in vitro,and phagocytosis test of the mice abdominal cavity macrophage test in vitro,and the immune response of vaccine with Newcastle disease in vivo.The results showed the activity of BS and MAP are significantly higher than that of KG30 in vitro,especially the BS was the best.However,the activities of MAP was the best of all in the further experiments in vivo.In conclusion,there was significant relationship between structure and function,structure has a significant effect on the the activity of the bursin like peptides,and the branch structure of bursin like peptide had the best valid enhancement to immunities.