Simultaneous determination of gallic acid and hesperidin contained in Xiaogu capsule by HPLC

Objective: To develop an HPLC method for simultaneous determination of gallic acid and hesperidin in Xiaogu capsule,in order to provide a simple,rapid and accurate method for quality control of the compound preparation of traditional Chinese medicine.Method: Xiaogu capsule was extracted with methanol heating reflux method.Synergi 4μ Hydro-RP 80A(4.6 mm×250 mm,5 μm) was adopted as the chromatographic column,with acetonitrile-0.04 mol·L-1 phosphate monobasic sodium solution(20∶80) as the mobile phase.The flow rate was 1.0 mL·min-1,the detection wavelength was 283 nm,and the column temperature was 25 ℃.Result: Under the conditions,gallic acid and hesperidin reached the baseline resolved peak,with a good linearity within the range of 21.6-216.0 mg·L-1(r=0.999 93)for gallic acid,and 4.5-45.0 mg·L-1(r=0.999 95) for hesperidin,respectively.Their average recoveries(n=9) were 101.5%(RSD 3.7%) and 94.7%(RSD 2.7%),respectively.The average contents of gallic acid and hesperidin contained in Xiaogu capsule were detected to 5.10% and 0.091 1%,respectively.Conclusion: The method established in this study can determine the content of gallic acid and hesperidin contained in Xiaogu capsule in a rapid and accurate manner,which provided reference for quality evaluation of the medicine.