Dynamic observation on rabbit ear hypertrophic scar formation with high frequency ultrasound biomicroscopy

Objective To dynamically observe the process of morphological changes of rabbit ear model of hypertrophic scar(HS) with high frequency ultrasound biomicroscopy(HFB).Methods Using Panoview β1500 HFB with 50 MHz high frequency probe,the thickness of HS was measured with ultrasound examination on the 1st,3rd,5th,7th,11th,16th,20th,28th,36th,60th and 90th day after establishment of rabbit ear HS models,respectively.HFB and histopathology were used to observe dynamic changes of the entire HS internal structure.Results The ventral side of each rabbit ear wound could produce excessive dermal scarring similar to human hypertrophic scar.HFB could observe the formation of HS clearly and dynamically.Significant direct correlation(r=0.996,P0.01) was found between HFB measurements and pathological measurements.Conclusion HFB can observe and quantitive analyze the complete rabbit ear hypertrophic scars and the internal structures,therefore can be used as an effective method of observation and measurement of HS.