Paternity investigation experience with a 40 autosomal SNP panel

Abstract With both the SNP for ID and the Kidd panels of autosomal SNPs available, we selected the 40 most informative and population-independent SNPs from both these sets for evaluation in paternity testing and as a prelude for forensic human identification. We used the published primer sequences and constructed PCR multiplexes for genotyping using the SNaPshot assay. Fifty trios and 50 duos previously analysed using conventional autosomal STR markers were re-analysed using the 40 SNPs. We report our findings regarding the practical use of these markers including unexpected mutations which impacted significantly on the use of this panel.