Characterization of hGH from patients with LTD by a human liver radio-receptor assay (RRA)

A RRA for hGH has been developed using the 100,000 g pellet from human liver tissue homogenate obtained from a donor of kidney transplantation. This assay is sensitive in the physiological range of hGH (1.9 - 30.0 ng/ml). Human prolactin and human placental lactogen did not compete with hGH on its receptor. Dilution curves parallel to the standard curve were obtained by receptor-active hGH found in the plasma of 7 LTD patients, four stimulated hGH healthy children and 2 acromegalics. Examples of results as hGH ng/ml are: