Tailoring the active site of chemzymes by using a chemogenetic-optimization procedure : towards substrate-specific artificial hydrogenases based on the biotin-avidin technology

The combination of chemical- with genetic-optimization strategies (i.e. chemogenetic) allows the production of artificial hydrogenases based on the biotin–avidin technology. In the spirit of enzymes, second-coordination-sphere interactions between the host protein (streptavidin) and the substrate (an olefin) allow fine-tuning of the selectivity to produce either R or S hydrogenation products.