Rat-Liver Cell Compartition as Revealed by Correlations Between Redox-Quotient Changes Following Alloxan Treatment, Starvation, Carbohydrate- and Fat-Diet

AbstractAs reported elsewhere (Feraudi, 1976a & b), we have studied the mathematical relations between metabolite concentrations in the rat liver at various redox states and expressed them algebraically. In the present work we have measured the liver-cell concentrations of lactate, pyruvate, glycerol 3-phosphate, dihydroxyacetone phosphate, malate, oxaloacetate, β-hydroxybutyrate, acetoacetate, 2-oxoglutarate, ribulose 5-phosphate as pentose phosphates, gluconate 6-phosphate, isocitrate, aspartate in untreated and treated rats (alloxan-diabetic, insulin-treated alloxan-diabetic or starved rats as well as rats fed on carbohydrate- or fat diet). Through analysis of the algebraic correlation between metabolite concentrations, we arrived at the following statements1. Under certain physiological conditions the concentration of some metabolites in one compartment determines their total quantity in the cell;2. NADP and NADPH are comparted within the cytosol;3. Reduced cosubstrate/oxidized cosubstrate ratios of s...