Cloning and Sequence Analysis of Gallus IL-2 Gene and Prediction of Protein Structure

According to GenBank in the IL-2 cDNA sequences, using Premier 5.0 software design a pairs of specific primer, the local Dorking inject 100 μg/ml Con A, total RNA was extracted from feeding 24 hours lymphocyte from spleen, by RT-PCR cloning colony IL-2 cDNA fragment. The DNA fragments were analyzed use DNAStar software between different species identity for comparison. Sequence analysis showed that IL-2 gene has an open reading frames for 432 nucleotide acids, encoding 143 amino acids and most of signal peptide, compared with published on Genbank, the nucleotide identity was 98.8%、31.2%、28.2%、27.3%、 30.6%、26.2%、31.7%、30.1%、30.8%、26.6%、30.6%. compared with published on Genbank, the Amino acids was 95.8%, 7.6%、7.6%、9.7%、7.6%、6.9%、10.4% 11.8%, 7.6%, 9.0%, 7.6%, 10.4% with cattle, horse, cat, sheep, bovine, canis, people, capra, mice, cow of IL-2 sequence comparison The gallus IL-2 gene was successfully cloned, there is a species-specific. And construct a based on further study the biological effects of IL-2 gene in particular the use of IL-2 enhance the immune effect of DNA vaccine.